Composite

Part:BBa_K3573001:Design

Designed by: Jungwoo Choe   Group: iGEM20_Korea_HS   (2020-10-10)


Single chain variable fragment with CPP recognizing RAS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Last year, Korea_HS team has modelled the structure of CPP-scFv(RAS) using the program Modeller. Modeller used searched the database and used similar structures (PDB ID: 5B3N, 6NJL, 5GS3, 6G8R and 3UYP) to build a homology model. Based on the results of the homology modelling, we have decided to synthesize the gene for CPP-scFv(RAS) for protein production and binding assays.

CPP (BBa_K3090000) was attached to scFv(RAS)(BBa_3573000) for entry into the cell.

Source

DNA was synthesized

References